Not all macromolecules behave alike. Your samples may have a different conformation than molecular standards or exhibit unexpected column interactions. As an absolute technique for determining molar mass and size in solution, SEC-MALS - the combination of size-exclusion chromatography with multi-angle light scattering – offers an advanced characterization technique and overcomes the many limitations of column calibration.
If you already analyze your samples by analytical SEC, you have probably overcome the most challenging part of SEC-MALS: finding the right column, mobile phase and other conditions that ensure good separation and recovery. Now you are ready to add MALS for absolute characterization and deeper understanding.
As shown in the example on the right, getting the correct molar mass with MALS is independent of molecular conformation and elution volume.
The DAWN® is the premier Multi-Angle static Light Scattering (MALS) detector for absolute characterization of the molar mass and size of macromolecules and nanoparticles in solution, offering the highest sensitivity and widest range of molecular weight, size and concentrations. It is most often used in conjunction with Size Exclusion Chromatography (SEC-MALS) or Field-Flow Fractionation (FFF-MALS) to determine distributions of mass, size and composition independent of column calibration by reference standards.
Included with each DAWN is a copy of ASTRA, the most versatile software package available for analysis of online multi-angle and dynamic light scattering. The DAWN and its companion Optilab dRI detector are the established benchmarks for MALS analysis, cited in thousands of peer-reviewed publications. With the addition of a ViscoStar differential viscometer or WyattQELS DLS module, the triple-detection system can analyze molecular conformation and sizes below the rg limit of 10 nm. The DAWN may also be used in batch (off-line) mode to characterize the weight-average molar mass, z-average size and second virial coefficient of unfractionated samples.
The miniDAWN® Multi-Angle static Light Scattering (MALS) detector performs absolute characterization of the molar mass and size of macromolecules and nanoparticles in solution, offering superb sensitivity over a wide range of molecular weight, size and concentration. miniDAWN performance for MALS analysis is exceeded only by the DAWN which covers a wider range of sizes and molecular weights with even better sensitivity. Three discrete photodetectors are spaced around the flow cell in an optical geometry, ensuring that measurements are made simultaneously over a broad range of scattering angles.
The miniDAWN is most often used in conjunction with Size Exclusion Chromatography (SEC-MALS) or Field-Flow Fractionation (FFF-MALS) to determine distributions of mass, size and composition, independent of column calibration by reference standards. The miniDAWN and its companion Optilab dRI detector are well-established for MALS analysis, cited in thousands of peer-reviewed publication. With the addition of a ViscoStar differential viscometer or WyattQELS module, the triple-detection system can analyze conformation and sizes below the rg limit.
The microDAWN® brings all the benefits of multi-angle light scattering (MALS) analysis to ultra-high performance liquid chromatography (UHPLC). Coupled to the Wyatt Technology’s microOptilab Refractive Index detector, a complete UHPLC-MALS system can be built to characterize proteins, polymers for solution properties of macromolecules, molar mass -independent of elution time; molecular size, conjugation, conformation, oligomeric states etc. UHPLC enables fast, efficient separation of biomolecular samples with improved resolution, higher throughput, less solvent and smaller sample consumption for analysis of precious biological samples. MALS eliminates the uncertainties of column calibration, molecular standards and non-ideal column interactions and affords extra-high sensitivity to aggregates.
Every microDAWN includes an embedded COMET (Cell Operation Maintenance Enhancement Technology ) for automated ultrasonic flow cell cleaning which helps in keeping baseline noise under check and achieving high sensitivity. The microDAWN fully integrates with the WyattQELS (Online DLS) for measurement of hydrodynamic radius of eluting macromolecules in the same flow cell of the MALS detectors guaranteeing dispersion-less correlation between molar mass, rms radius and hydrodynamic radius