Composition-Gradient, Multi-Angle Static Light Scattering (CG-MALS) is a powerful technique for characterizing a wide range of biomolecular interactions. Composition-Gradient Multi-Angle Light Scattering (CG-MALS) employs a series of unfractionated samples of different composition or concentration in order to characterize a wide range of macromolecular interactions. No special modifications (e.g., sample tagging or immobilization procedures) are necessary: samples are unlabeled and entirely in solution.
CG-MALS employs a Calypso® connected to a MALS instrument and optional concentration detector. The Calypso performs sample preparation and delivery, combining up to three different solution in precise mixing ratios and injecting into the detectors.
Accurate compositions result from setting the Calypso's pumps to appropriate flow rate ratios, all controlled from the CALYPSO™ software through an intuitive GUI. Mixing is achieved by dispensing the solutions simultaneously from all three pumps via a static mixer.
Reliable light scattering measurement require the samples to be degassed and filtered. The Calypso incorporates built-in, low-volume degassers and filter housings, connected via PEEK tubing and finger-tight fittings. Even the filter housing is finger-tight, making use of a proprietary design for maximum convenience.
The layout and design were fine-tuned to reduce sample use while enhancing automation and maintaining compatibility with the DAWN and miniDAWN, the ultimate in MALS detectors. Special features of the Calypso include:
Recognizing that CG-MALS is unfamiliar territory for most users, we've also developed Tutorials, Application Guides, Method Templates, and additional support literature and training to help you get started and keep you on track. Make the most of this amazingly powerful technique - we're here to help at every turn.