Unprecedented Resolution and Peak Shape of Basic Compounds

Even for LC columns that claim to be designed for basic compounds, adequate resolution often cannot be obtained due to problems such as leading of highly polar basic compounds, peak shape deterioration of acidic compounds, or long equilibration time required for low ionic strength acidic mobile phase. All of these issues have been solved with Shim-pack Arata that was specifically designed to give unmatched peak shape for basic compounds.

Unmatched Peak Shape Elegantly Designed for A Wide Range of Compounds

Unmatched peak shape of basic compounds could be achieved while maintaining good peak shape for acidic compounds with Shim-pack Arata LC columns. Even with low ionic strength acidic mobile phase, such as 0.1% formic acid containing mobile phase, excellent peak shape of both amitriptyline (a basic compound) and benzoic acid (an acidic compound) could be achieved.

Excellent Separation Performance for Peptides Even with Weak Ion Paring Acids

In order to obtain good peak shape of peptides under reversed phase chromatography, TFA containing mobile phases are frequently used which the ion pairing effect is relatively strong. However, TFA could cause ion suppression in LC/MS (/MS) analysis. Excellent peak shape and separation performance for peptides could be achieved on the Shim-pack Arata LC column even with 0.1 % formic acid (weak ion paring acid) containing mobile phase.

Typically, in order to obtain good peak shape of peptides under reversed phase chromatography, TFA containing mobile phases are frequently used which the ion pairing effect is relatively strong. However, TFA could cause ion suppression in LC/MS(/MS) analysis. Excellent peak shape and separation performance for peptides could be achieved on the Shim-pack Arata LC column even under formic acid (weak ion paring acid) containing mobile phase conditions, which are suitable for LC/MS(/MS) without the use of typical ion pairing agents.

Increased Assurance of Peptide Analysis

In order to ensure lot-to-lot reproducibility in peptide analysis, each lot of Shim-pack Arata Peptide C18 material is tested using a mixture of peptide standards in addition to the standard Shim-pack Arata C18 lot QC test. This test is carried out under severe condition using 0.1 % formic acid mobile phase to help ensure consistent column performance for requirements of customers under regulated requirements.

High Peptide Recovery enabled by Shim-pack Arata technology

Peptides are known to show non-specific adsorption to particles of LC columns. Since the adsorption of peptides to particles is minimized, high recovery of peptides is ensured and excellent peptides analysis can be provided by Shim-pack Arata Peptide C18 column.

Analytical Conditions
LC System : Nexera X2 M30A (STD Cell)
LC Column : Shim-pack Arata Peptide C18 (2.0 × 150 mm I.D., 2.2 μm)
Core shell Peptide C18 (2.1 × 150 mm I.D., sub 2 μm)
Core shell C8 (2.1 × 150 mm I.D., sub 4 μm)
Mobile Phase A : 0.1 % HCOOH in H2O
Mobile Phase B : 0.1 % HCOOH in CH3CN
Gradient : 2 %B (0-5 min)􀋠45 %B (20 min)􀋠 100 %B (20.01 – 25 min)
􀋠 2 %B (25.01 – 30 min)
Flow rate : 0.2 mL/min
Detection : 214 nm
Column Temp. : 40 °C
Injection volume : 5 μL
Sample : Myoglobin tryptic digest
Vial : TORAST-H Bio Vial