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LC/MS/MS Method Package for Mycotoxins

Mycotoxins are highly toxic chemical byproducts from fungi commonly found in crops, and residual content in foods are regulated the world over. This method package provides simultaneous multi-component analysis conditions for mycotoxins subject to the regulations in Japan, the USA, the EU, and China.

Analytical Methods for 27 Mycotoxins

This package contains seven different analytical method conditions and fully optimized MRM transitions for a total of 27 different mycotoxins. Rapid analytical methods are included for a 25 mycotoxin screen, 16 mycotoxins using high-sensitivity conditions, 10 mycotoxins using APCI, and specialized methods for trichothecenes, fumonisins, aflatoxins, and patulin.

Analytical Method for 25 Mycotoxins in 15 Minutes
HPLC Conditions
  • Column : Mastro™ PFP2 (Shimadzu GLC)(2.1 mmI.D. × 150 mmL, 3 µm)
  • Mobile Phase A : 10 mmol/L aqueous ammonium acetate
  • Mobile Phase B : Methanol (2 % acetic acid)
  • Flowrate : 0.4 mL/min
  • Column Temp. : 40 °C
MS Conditions
  • Ionization Mode : ESI (+/-)
  • Nebulizer Gas Flowrate : 2 L/min
  • Drying Gas Flowrate : 10 L/min
  • Heating Gas Flowrate : 10 L/min
  • Interface Temp. : 300 °C
  • DL Temp. : 250 °C
  • Heat Block Temp. : 500 °C
High-Speed Simultaneous Analysis of 25 Mycotoxins
LC/MS/MS Method Package for Mycotoxins
Compound List for Each Method

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