These methods target culture medium components and metabolites secreted by cells. This culture medium analysis platform enables the simultaneous analysis of up to 95 components, the highest number of target analysis components* that can be analyzed by such a platform. Such features make it possible to acquire detailed data concerning cell culture profiles.
Compounds such as amino acids and vitamins are commonly analyzed by each compound group, which makes analysis of a culture medium time-consuming. By providing conditions for efficient and simultaneous multi-component analysis, this method package enables simultaneous analysis in 17 minutes.
Pre-set analysis conditions fully utilize the capabilities of LC-MS/MS for analyzing trace components such as vitamins. In addition, since there is no saturation of the signal with high-concentration components, such as glucose or amino acids, it is possible to measure a variety of culture medium components using the same vial.
Shimadzu Method Packages deliver conditions for efficient and simultaneous multi-component analysis. They enable the user to quickly and easily implement complex methods without costly and laborious method development by providing sample preparation protocols, LC separation conditions, and MS acquisition parameters.