Comprehensive 2D-LC methodology is a paradigm shift in liquid chromatography separation analysis. By combining two independent separation modes orthogonally in combination with a dual-loop/dual-valve alternate switching design, the highest possible peak capacity is achieved for LC chromatography separation. The new Nexera-e enables separation of even the most complex mixtures, providing a new level of knowledge and understanding of sample analytes. This is especially beneficial to the analysis of pharmaceutical impurities, proteolytic digests, food extracts, natural products and synthetic polymers.
Comprehensive 2D-LC fractionates the 1st-dimensional eluent and successively separates the fraction on-line with the 2nd-dimensional system. Therefore, the 1st-dimensional system intersects with the 2nd-dimensional system at the dual valves and dual-loops. With valve switching, these loops continue the fractionation of the eluent from the 1st-dimensional system and the injection of the fraction to the 2nd-dimensional system alternately.