The microDAWN brings all the benefits of multi-angle light scattering (MALS) analysis to ultra-high performance liquid chromatography (UHPLC) . Coupled to the Wyatt Technology’s microOptilab Refractive Index detector, a complete UHPLC-MALS system can be built to characterize proteins, polymers for solution properties of macromolecules, molar mass -independent of elution time; molecular size, conjugation, conformation, oligomeric states etc. UHPLC enables fast, efficient separation of biomolecular samples with improved resolution, higher throughput, less solvent and smaller sample consumption for analysis of precious biological samples. MALS eliminates the uncertainties of column calibration, molecular standards and non-ideal column interactions and affords extra-high sensitivity to aggregates.
Every microDAWN includes an embedded COMET (Cell Operation Maintenance Enhancement Technology ) for automated ultrasonic flow cell cleaning which helps in keeping baseline noise under check and achieving high sensitivity. The microDAWN fully integrates with the WyattQELS (Online DLS) for measurement of hydrodynamic radius of eluting macromolecules in the same flow cell of the MALS detectors guaranteeing dispersion-less correlation between molar mass, rms radius and hydrodynamic radius