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Eclipse™ DualTec™ FFF System

Flow Field-Flow Fractionation is a uniquely versatile means of size-based separations, capable of fractionating and characterizing proteins and aggregates, liposomes, emulsions, viral particles, polysaccharides, nanoparticles, polymer latex particles, colloidal soil suspensions, chemical mechanical polishing slurries, and many more - with no stationary phase and almost no shear.

The Eclipse™ DualTec™ system offers both Hollow-Fiber Flow-FFF (HF5) and Asymmetric Flow-FFF(AF4) techniques. Both may be integrated into one instrument and coupled seamlessly to the most advanced light scattering detector for determination of molar mass and nanoparticle size, Wyatt's DAWN® HELEOS® II.

Key Benefits

  • Robust separation of macromolecules and nanoparticles from 1 nm - 1000 nm with excellent resolution
  • Typical analysis times 5 - 30 minutes
  • No stationary phase - open channel separation with minimal shear and surface interactions
  • Versatile choice of separation channels, permitting sample loads from nL to mL, including semi-preparative FFF
  • Optimize separation methods to enhance resolution or size range simply by adjusting flow ratios, even programmatically during a run
  • Fractions can be collected and used for off-line analysis (with electron microscopy, MS, ICP-MS, ELISA, etc.)

Complement SEC-MALS

Eclipse FFF systems add to and complement size exclusion chromatography(SEC) for macromolecular separations without shear or adverse, non-ideal column interactions. The Eclipse™ DualTec™ allows one to take full advantage of the favourite conventional SEC and AF4/HF5 separations in one convenient package. FFF-MALS and FFF-DLS performed with an Eclipse™ DualTec™, DAWN HELEOS II and an embedded WyattQELS DLS detection module offer excellent resolution and accuracy in analysis of size distributions. Particle counts and sizes of viruses and virus-like particles estimated by FFF-MALS match closely TEM-based counts.

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