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NUCLEOCOUNTER® NC-100™ Mammalian Cell Counter

The NucleoCounter® NC-100™ is an image cytometer based on fluorescence from the fluorescent dye, propidium iodide (PI). The NC-100™ eliminates human subjectivity and can count even the most aggregated mammalian cells using the NucleoCassette™. The NucleoCassette™ itself is calibrated and severely limits user interference making the NC-100™ very suitable for GMP.

The NucleoCounter® NC-100™ concept offers determination of cell viability in addition to the total count of cells in the sample. Determination of viability is based on the “PI-exclusion” method. The cell viability of a sample is determined using the total cell count and the count of non-viable cells as shown below. When a sample is mixed with Reagent A100 and Reagent B a lysis of the viable cell membrane takes place rendering all the cell nuclei susceptible to staining with propidium bromide (PI) resulting in a total cell count. Non viable cells however are permeable without treatment and are therefore stained directly with PI, resulting in a non-viable count.

Benefits

  • Easy operation
  • 30 sec.analysis time
  • No cleaning or calibration
  • Maintenance and service free
  • Excellent reproducibility
  • Safe sample handling and disposal
  • Excellent for aggregated cells
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NUCLEOCOUNTER® NC-100™
Mammalian Cell Counter