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Gemini EM Microplate Reader

The Gemini EM Microplate Reader with dual monochromators lets determination of optimal excitation and emission settings for the fluorescence intensity assays. The system helps in creation of custom fluorescence assays without having to purchase expensive filter sets and also easy switch over between the top and bottom reading modes for solution- and cell-based assays. Comprehensive validation package for the system include : SpectraTest® ABS1, FL1 and LM1 Validation Plates for hardware validation of absorbance, SoftMax Pro Software validation package, Software tools for FDA 21 CFR Part 11 compliance.


  • Read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes
  • Eliminate the need for identifying, purchasing, and changing out filters for wavelength optimization with dual monochromators for excitation and emission wavelength selection between 250 and 850 nm
  • Measure fluorescence from solutions or cell-based assays with the best optical sensitivity by switching between top and bottom fluorescence reading
  • Up to 4 wavelength pairs can be read for endpoint and kinetic measurements
  • Well scanning to report a fluorescent measurement from a single point in the center of a microplate well to multiple points across a tissue culture well

Measuring Accurately and Reproducibly

  • Avoid losing high signals to detector saturation and photobleaching after repeated reads to find the correct reader settings with AutoPMT System electronics
  • Compare relative fluorescence units (RFUs) between samples, like with no other fluorometer, allowed by a unique calibration against an internal standard
  • Monitoring of temperature-sensitive reactions with consistent temperature regulation from ambient to 45oC
  • Thorough mixing of samples in microplate wells by user-programmable Automix feature
  • Gentle movement of cell or other aggregates in the microplate with a slow carriage speed option
  • Seamless automation coupling with the StakMax Stacker, Molecular Devices own integrated microplate stacker designed exclusively for the SpectraMax Reader
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