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Axopatch 200B Amplifier

Widely accepted as the gold-standard for ultra low-noise patch clamp recordings, the Axopatch™ 200B Capacitor Feedback Patch Clamp Amplifier is the premier microelectrode amplifier for ultra-low noise patch clamp recordings. The system incorporates capacitor-feedback technology and active headstage cooling for single-channel recording, while resistive-feedback circuitry is used for two different ranges of whole-cell recording.

  • Actively cooled capacitor-feedback headstage reduces thermal noise for signal-to-noise ratios close to the theoretical limits of physics
  • Integrating headstage mode for measuring sub-picoamp currents to enable high-fidelity single-channel recordings from cell membranes and artificial bilayers
  • Resistive headstage mode for larger currents and noise levels in whole-cell recordings
  • Slim headstage design for easier access to the preparation on a crowded microscope stage
  • Series resistance compensation: Prediction helps achieve the desired command level faster by transiently supercharging the membrane potential
  • Series resistance compensation: Correction improves the bandwidth of the recording by eliminating the error introduced by the voltage drop across the series resistance
  • Dual-speed current clamp optimizes speed and stability through better matching of the clamp algorithm to experimental parameters
  • Integrated seal test in voltage- and current-clamp mode for easy monitoring of membrane and cell health parameters
  • Dual command potentials add flexibility by processing input signals from two different sources
  • Selectable holding command input with two internal range settings and an external input enable convenient control of the output waveform
  • Pipette capacitance compensation neutralizes the contribution of the glass pipette and the pipette holder to the capacitance of the circuitry
  • Cell capacitance compensation corrects for the capacitance of the cell membrane in whole-cell recordings
  • Selectable output gain with 10 settings to scale the output signal to the desired level
  • Leak subtraction for correction of leak currents without software-based algorithms
  • Sophisticated 4-pole Bessel low-pass filter acts as anti-aliasing filter and can be used to pre-condition the output signal
  • Configurable panel meter displays user-selectable parameters in real time for quick monitoring of the experiment
  • Zap facilitates patch rupturing by applying a voltage pulse for a user-defined duration
  • Two included pipette holders of different barrel lengths accept glass pipettes in most standard dimensions
  • User guide written by scientific consultants, with the assistance of Axon Instruments staff, for straightforward start-up and as an in-depth reference

In combination with a Digidata® 1550B Data Acquisition System and pCLAMP™ Software, the Axopatch 200B Amplifier forms the foundation of a patch clamp setup like those used in many electrophysiology labs around the world. Telegraphing between the software and the amplifier ensure correct scaling and logging of experimental parameters. The Clampex Data Acquisition Module, which is part of the pCLAMP Software, makes optimal use of the many advanced features of the Axopatch 200B Amplifier.

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