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Axoclamp 900A Amplifier

Axoclamp 900A Microelectrode Amplifier is a complete microelectrode current clamp and voltage clamp amplifier, useful for a wide range of intracellular microelectrode recording techniques. The Axoclamp 900A Microelectrode Amplifier provides the following modes of operation:

  • I-Clamp: Current clamp using two independent bridge amplifiers for voltage measurements
  • DCC: Discontinuous current clamp for accurate voltage measurements, even when the electrode resistance changes
  • TEVC: High-compliance two-electrode voltage clamp for Xenopus oocytes and mammalian cells
  • dSEVC: Discontinuous single-electrode voltage clamp for small cells with large currents
  • HVIC: High-voltage current clamp for extracellular applications such as iontophoresis

Salient Features

  • Five modes of operation provide the broadest range of electrophysiological applications
  • Software control of all amplifier settings for automated optimization features, reduction of manipulation steps, and convenient, reliable telegraphing
  • Optional SoftPanel allows amplifier control using knobs and buttons for a more conventional feel to amplifier control
  • Large output compliance range (±180 V in TEVC and HVIC modes) enables passing larger currents and ensures faster clamp speeds
  • Wide AC voltage clamp gain ranges in TEVC and dSEVC modes for excellent voltage control
  • Space-saving headstage design for easier access to the preparation on a crowded microscope stage
  • New dSEVC design with more stability and faster clamp provides an excellent alternative to standard continuous single-electrode voltage clamping
  • Pipette Capacitance Compensation neutralizes the contribution of the glass pipette and the pipette holder to the capacitance of the circuitry
  • Automatic oscillation detection prevents damage to a cell when electrical feedback occurs
  • Selectable output gain with 11 settings to scale the output signal to the desired level
  • Audio monitor for acoustic tracking of the signal while you are busy looking down a microscope
  • Fine-tunable 4-pole Bessel and Butterworth low-pass filters act as anti-aliasing filters and can be used to pre-condition the output signal
  • Buzz feature with a hand-held remote switch facilitates breaking through cell membranes
  • Two included pipette holders of different barrel lengths accept glass pipettes in most standard dimensions
  • User guide written by scientific consultants, with the assistance of Axon Instruments staff, for straightforward start-up and as an in-depth reference

In combination with a Digidata 1550B Data Acquisition System and pCLAMP Software, the Axoclamp 900A Amplifier forms the core of a recording setup like those used in many electrophysiology labs around the world.

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