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Assay Kits

EarlyTox Cardiotoxicity Kit

The EarlyTox™ Cardiotoxicity Kit provides a fast, simple, and reliable fluorescence-based method for identifying cardiotoxic compounds in a biorelevant assay using stem-cell derived cardiomyocytes. Leveraging our proprietary masking technology with our novel calcium sensitive indicator, researchers can:

  • Eliminate cardiotoxic compounds and identify potential drug candidates
  • Measure 384 samples in minutes rather than hours
  • Minimize non-specific effect of dye on beat characteristics
  • Reduce well-to-well variation and improve data quality
  • Largest signal dynamic range available

Benchmark against three reference compounds: isoproterenol, sotalol and propranolol

EarlyTox Cell Integrity Kit

The EarlyTox Cell Integrity Kit is an optimized set of reagents that simplifies the identification of live and dead cells. It can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms, including apoptosis and necrosis. The kit is designed to work with many cell types, both adherent and non-adherent. The protocol's simple workflow and reagent performance make it amenable to high-throughput screening.

  • Higher signal allows shorter exposure times for fast results
  • Designed to work with many cell types

Streamlined image acquisition and analysis on a single system

EarlyTox Cell Viability Assay Kits

Cell viability assays are critical to a broad spectrum of research areas ranging from investigation into the mechanisms of cell death to the development of new therapeutics targeting apoptosis in diseases. One of the most popular detection technologies for cell viability is a fluorescence microplate reader. The EarlyTox cell viability assay kits have been optimized for highly sensitive and accurate microplate detection which provides a complete solution for monitoring cell viability.

  • Optimal data quality for microplate reader assay
  • Simplified, homogeneous experimental protocol

Preconfigured SoftMax Pro Software protocols

SpectraMax Quant dsDNA Assay Kits

The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use.

  • Three kits covering a wide range of dsDNA concentrations
  • Highly selective for dsDNA over ssDNA or RNA
  • High tolerance for contaminants
  • Optimized for fluorescence microplate readers
  • Simplified data acquisition and analysis with preconfigured protocols in SoftMax Pro Software

CatchPoint SimpleStep ELISA kits

Fluorescent ELISA kits developed in collaboration with Abcam Don’t miss a thing with our fluorescent ELISA kits.

CatchPoint® SimpleStep ELISA® kits have been developed using a fluorescent substrate to provide improved linearity over an extended dynamic range, providing better quantification at both the lower and the upper end of the curve. These kits developed in collaboration with Abcam, are currently sold and supported by Abcam.

FLIPR Calcium Assay Kit

Built as the most comprehensive portfolio of calcium reagents, the kits deliver pre-optimized, homogeneous, fluorescence-based formulations to expedite assay development and screening of GPCR and ion channel targets. FLIPR Calcium Assay Kits provide the most comprehensive method for detecting intracellular calcium changes in a simple and reliable homogeneous assay format. Select from six formulations to complement your target of interest.

FLIPR Assay Kits employ a quenching dye to reduce background fluorescence and improve the signal-to-noise ratio. The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices, through the purchase of FLIPR Assay Kits.

Key Assay Features and Benefits

  • Universal mix-and-read protocol accelerates assay workflow and increases throughput
  • Superior signal-to-noise ratio facilitates confirmation of endogenous or transiently transfected receptor activity during assay development
  • Pre-optimized and validated protocols for the FLIPR® Tetra and FlexStation® Systems ensure you can navigate both routine and unconventional cell lines and targets

Fura-2 QBT Calcium Kit

The Fura-2 QBT Calcium Kit eliminates the cause of data variability and reduces the number of steps compared to conventional wash protocols using Fura-2. Leveraging our proprietary masking technology with the industry standard Fura-2 ratiometric calcium indicator, researchers can:

  • See largest Fura-2 signal window available
  • Eliminate wash artifacts and increase throughput with homogeneous assay
  • Minimize the impact of uneven dye loading and leakage on results
  • Interrogate low-density, weakly or non-adherent cells using no-wash protocol
  • Assay on FlexStation® 3 and FLIPR® Tetra Systems
  • Reference thousands of citations where Fura-2 has been used for cell lines and target

CatchPoint cAMP Fluorescent Assay Kit

cAMP Fluorescent Assay Kit's high-affinity reagents are optimized for sensitivity and precision in applications where cAMP levels are low. A single wash step removes unbound material prior to the development step, so the assay is very resistant to interference from colored or fluorescent test compounds. The proprietary Stoplight Red substrate is used to generate a stable fluorescent readout, allowing the assay to be read in as little as 10 minutes or up to 24 hours after substrate addition.

Benefits of CatchPoint cAMP Fluorescent Assay Kit:

  • High affinity reagents are optimized for sensitivity when cAMP levels are low, ensuring results when other assays fail.
  • Robust format resists interference from colored or fluorescent compounds, improving reliability of results.
  • Rapid signal development (< 10 min) and no termination step give users a flexible read time that is compatible with automation and high-throughput screening.
  • Quantitative method allows accurate determination of cAMP in samples.

CatchPoint cGMP Fluorescent Assay Kit

The CatchPoint® cGMP Fluorescent Assay Kit's high-affinity reagents are optimized for sensitivity and precision in applications where cGMP levels are low. A single wash step removes unbound material prior to the development step, so the assay is very resistant to interference from colored or fluorescent test compounds. The proprietary Stoplight Red substrate is used to generate a stable fluorescent readout, allowing the assay to be read in as little as 10 minutes or up to 24 hours after substrate addition.

Benefits of CatchPoint cGMP Fluorescent Assay Kit:

  • High affinity reagents are optimized for sensitivity when cGMP levels are low, ensuring results when other assays fail.
  • Robust format resists interference from colored or fluorescent compounds, improving reliability of results.
  • Rapid signal development (< 10 min) and no termination step give users a flexible read time that is compatible with automation and high-throughput screening.
  • Quantitative method allows accurate determination of cGMP in samples.

FLIPR Potassium Assay Kit

The FLIPR Potassium Assay Kit exploits the permeability of thallium ions (Tl+) through both voltage- and ligand-gated potassium (K+) channels. In this assay, a novel, highly-sensitive Tl+ indicator dye is utilized which produces a bright fluorescent signal upon the binding to Tl+ conducted through potassium channels. The intensity of the Tl+ signal is proportional to the number of potassium channels in the open state; therefore it provides a functional indication of the potassium channel activities. In addition, one of our proprietary masking dyes is employed to further reduce background fluorescence for improved signal/noise ratio.

  • Functional measurement of potassium channel activity in a cell-based assay
  • Homogenous no-wash protocol reduces well-to-well variation and simplifies the workflow
  • Expanded signal window compared to non-homogenous assay
  • Rapid procedure with less hands-on time

FLIPR Membrane Potential Assay Kits

Each homogeneous assay kit utilizes a proprietary indicator dye and quencher combination to maximize cell line/channel/compound applicability, while eliminating causes of data variability. This unique formula responds 10 times faster and has greater temperature stability than traditional dyes, providing high quality screening data that shows good correlation with manual patch clamp assays.
Because ion channel activity is highly sensitive and potentially impacted by subtle chemical changes, two FLIPR® Membrane Potential Assay Kits (Red and Blue) are available to select the optimal conditions for your delicate ion channel targets. Both formulations utilize Molecular Devices proprietary quench technology to enhance signal windows and yield acceptable Z-scores to screen a variety of targets, including TRP, ligand-, cyclic nucleotide- and voltage-gated channels.
Unlike traditional dyes such as DiBAC, FLIPR Membrane Potential Assay Kit detects bidirectional gradient changes so you can monitor both variable and control conditions within a single experiment. We recommend evaluating both assay kits to discern which formulation is right for your target.

SpectraMax Glo Steady-Luc Reporter Assay Kit

Reporter gene assays are an important tool for biomedical and pharmaceutical researchers to monitor cellular events associated with gene expression, gene regulation, and signal transduction. Firefly luciferase, one of the most commonly used reporters, produces light by catalyzing a bioluminescence reaction that oxidizes D-luciferin in the presence of oxygen and ATP.

The application of luciferase-based reporter assay using luminescence microplate readers has become increasing popular for high-throughput analysis of gene expression as well as drug discovery applications. The SpectraMax Glo Steady-Luc Reporter Assay Kit is designed for optimal performance on microplate readers with high throughput enabled by both the microplate format and assay signal stability.

  • Glow-type luminescence provides an extended signal time window enabling batch processing of plates in screening assays
  • Optimized for luminescence microplate readers
  • Simplified data acquisition and analysis with preconfigured protocols in SoftMax Pro Software

SpectraMax DuoLuc Reporter Assay Kit

The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells at a great price.

  • Flash-type luminescence with excellent sensitivity
  • Expression measurement in as few as ten cells per well
  • Improved accuracy of results by normalizing reporter expression to a control
  • Optimized for SpectraMax® i3x, iD3, or L microplate readers with injectors
  • Simplified data acquisition and analysis with preconfigured protocol in SoftMax® Pro Software
  • Support for your complete solution of reader, software, and assay

QBT Fatty Acid Uptake Assay Kit

Conventional protocols for monitoring fatty acid uptake using radioactivity often require cell lysis and processing at very low temperature, making them expensive, slow and unsuitable for high throughput screening. Other fluorescence-based protocols generally require the use of low throughput fluorescence activated cell sorter (FACS) instrumentation, or require cell washing, which can severely compromise the integrity of the fragile adipocytes used in these assays.

The QBT Fatty Acid Uptake Assay uses a BODIPY®-dodecanoic acid fluorescent fatty acid analog coupled with our proprietary quench technology. The BODIPY label provides an ideal long chain fatty acid analog that behaves much like natural fatty acids and is a known substrate for fatty acid transporters.

  • One-step, homogeneous assay format enables high-throughput screening
  • Fluorescent assay with proprietary quench technology allows users to monitor real-time uptake kinetics in live cells
  • Replaces cumbersome and time-consuming fluorescent and radioactive assay protocols
  • The elimination of radioactive compounds reduces disposal costs and eliminates safety risks associated with radioactive assays

Neurotransmitter Transporter Uptake Assay Kit

Until recently, radioactively labeled compounds were used to measure serotonin, norepinephrine and dopamine transporter uptake. With the introduction of the Neurotransmitter Transporter Uptake Assay Kit, researchers now have a tool to screen for live-cell kinetic uptake for these three key neurotransmitters.

  • Homogeneous, live-cell fluorescent assay replaces radioactive assays, reducing disposal costs and eliminating safety risks associated with radiolabeled assays
  • One-step, homogeneous assay format is amenable to automation and high-throughput screening
  • Two assay setup options offer greater flexibility: endpoint mode for high-throughput applications, or real-time kinetic mode for mechanistic studies

ScanLater Western Blot Assay Kit

The ScanLater™ Western Blot Assay Kit is a time-resolved fluorescence (TRF)-based assay optimal for quantitating as little as femtogram protein samples. Using this novel western blot method, researchers can:

  • Time-resolved fluorescence detection
  • Europium-labeled goat anti-rabbit, goat anti-mouse, donkey anti-rat, donkey anti-goat or streptavidin labeled antibodies
  • Supports nitrocellulose and PVDF membranes
  • 340/80 nm excitation with 616/10 nm emission range
  • Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers
  • Optimized for use with the enhanced TRF detection module for the SpectraMax iD5 Multi-Mode Microplate Reader
  • Eliminate time-dependent substrate addition steps
  • Sustain blot signal stability for at least one month
  • Enhance assay sensitivity using TRF method to reduce background noise
  • Maintain femtogram to picogram protein sensitivity similar to traditional western blot detector

Contaminant Detection Assays

Two contaminant detection assay kits are available under the Threshold brand: Threshold® Immunoligand Assay (ILA) and Threshold® Total DNA Assay

Threshold Immunoligand Assay measures a broad range of analytes such as proteins, peptides, sequence-specific DNA and microorganisms for biopharmaceutical development and production. Samples can range from fermentation supernatants, samples from a purification process, and other biochemical preparations to serum or other bodily fluids. Assays can be developed using a sandwich or competitive format depending on the size and characteristics of the analyte to be measured.

Threshold Total DNA Assay quantitatively measures picograms of single-stranded DNA. Unlike hybridization techniques which require DNA probes or primers with a specific nucleic acid sequence, the Threshold Total DNA Assay measures DNA with broad sequence specificity. This enables the Threshold System to measure host-cell DNA as well as other DNA that might have been introduced during the biopharmaceutical manufacturing process. In addition, since the assay does not require sequence-specific probes or primers, it can easily be applied to different samples purified from different host organisms.

Enzyme - IMAP Assays

Based on the specific, high-affinity interaction of phospho groups with trivalent metal-containing nanoparticles (beads), IMAP is a generic, non-antibody-based platform to assess kinase, phosphatase, and phosphodiesterase activity. An enzyme reaction is performed using fluorescently labeled substrate. Addition of the IMAP Binding System stops the enzyme reaction and initiates binding of the beads to phosphorylated substrates. Binding of the substrate to the beads, which correlates to enzyme activity, can be detected using either FP or TR-FRET as a readout.

IMAP Progressive Binding System

IMAP Progressive Binding System lets researchers optimize their FP and TR-FRET assay conditions for each substrate used. The system consists of Progressive Binding Buffer A and Progressive Binding Buffer B, along with Progressive Binding Reagent. The two buffers and the reagent can be combined in different proportions according to the acidic character of the substrate choice, desirable ATP concentrations and background parameters.

IMAP Substrates

Molecular Devices offers a wide range of validated substrates and calibrators. Substrates may be used with the enzymes for which they were originally validated, or as potential substrates for other enzymes.
          • Validated IMAP substrates with optimized binding conditions ensure the best performance when using the IMAP platform
          • Substrates come in two sizes and can be used with both IMAP FP and IMAP TR-FRET
          • Dozens of different substrates have been validated with over 100 enzymes.Many substrates are available with either red or green fluorescent label,  enabling multiplexing and providing a way to address issues arising from compound autofluorescence

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