spinco biotech linkedin pageLinkedin spinco biotech youtube channelYoutube spinco biotech facebook pageFacebook spinco biotech twitter pageTwitter spinco biotech Instagram pageInstagram

MetaMorph Microscopy Automation and Image Analysis Software

MetaMorph Microscopy Automation and Image Analysis Software

The MetaMorph® Microscopy Automation and Image Analysis Software automates acquisition, device control, and image analysis. It easily integrates dissimilar fluorescent microscope hardware and peripherals into a single custom workstation. The software offers many user-friendly application modules for biology-specific analysis. Two complementary packages, MetaFluor® software for fluorescence ratio imaging, and MetaVue® software for basic image acquisition and processing, are included in the portfolio.

Support third-party microscopes

MetaMorph software works with many commercially available microscopes, laser launches, TIRF optics, and can be enabled on previously-installed imaging systems compatible with MetaMorph software.

Measure fluorescence ratio imaging

MetaFluor® Fluorescence Ratio Imaging Software is designed for single or multi-wavelength intracellular ion measurements to provide greater insight to ion exchange and intracellular function.

Document and analyze images

MetaVue® Research Imaging System is  single, easy-to-use software application for acquiring and processing images, performing graphics functions, and archiving and retrieving images.

An Integrated Imaging System for Maximized Control

MetaMorph software provides high-end control for devices including microscopes, filter wheels, shutters, cooled CCD cameras, video cameras, monochromators, focus motors and Piezo electric focus devices, motorized stages, digital and serial input/output, and robotic devices.   In addition to X and Y dimensions, you can acquire and display:

  • Z-axis or multiple focus series (Z dimension)
  • Multiple fluorochromes (Wavelength dimension)
  • Time lapse (Time dimension)
  • Multiple stage positions (Stage dimension

Developed in conjunction with leading bioscience researchers, MetaMorph software offers tools for imaging applications such as:

  • Multi-dimensional imaging • 3D deconvolution • 3D reconstruction • Colocalization and brightness measurements • Particle tracking and motion analysis • Fluorescence, FRET, FRAP, and FISH • Morphometry • Multi wavelengths cell segmentation • Neurite outgrowth • Angiogenesis tube formation • Time-lapse • Z-series • And more

Observe changes over time

Intensity over time measurements are important in studies such as protein motility, FRAP, FRET, and protein-protein interactions.  MetaMorph software facilitates time lapse acquisition by offering streaming as an acquisition option. With the appropriate devices, our patented streaming allows you to acquire at the maximum rate of the camera.  Another feature for time lapse is the Live Replay option. With appropriate devices and when viewing live images, you can press a key when an interesting event occurs and capture a stack containing some past history of the event as well as some data after the event happened  

Single time point of a time lapse series. Dendritic cells were surface labeled with an antibody to MHC class one and DRAC 5 to stain the nucleus. A data stack was collected using MetaMorph software.

Dendritic cells were loaded with FURA 2 AM and a scrape was made across the field of view using a fine micropipette tip. Following the scrape, the cells were left to stabilize for 10 minutes and then imaged using a Nikon 2000e2 microscope and MetaMorph software. The cells were poked with a micro injection tip. A calcium flux is seen to radiate out from the original cell across the dish and the flux is carried down the tunneling nanotubules. Results show that the flux did not cross the gap where the scrape occurred. Dendritic cells loaded with FURA 2 AM

Simon C. Watkins Ph.D., University of Pittsburgh School of Medicine, Pittsburgh, PA 15213 Images courtesy of Simon C. Watkins Ph.D., Department of Cell Biology and Physiology and Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213.

Count, classify and measure multiple cell parameters

Canned, application-specific analysis modules are available for MetaMorph software: Angiogenesis, Cell Cycle, Cell Health, Count Nuclei/Cell Scoring, Granularity, Live/Dead, Mitotic Index, Monopole Detection, Multi Wavelength Cell Scoring and Neurite Outgrowth Application Modules. These modules provide users with a range of tools to automate processing and analysis of cellular images. No special microscopy or image analysis knowledge is required. Cellular segmentation and measurements are generated without the need for programming.

MetaMorph software’s morphometry tools allow you to choose over 100 different parameters for morphometric measurement or classification of cells in monochrome or color images. Measure all the objects in your image or define filters which restrict the measurements to objects that meet specific criteria. 

Large-scale chromatin decondensation and recondensation regulated by transcription from a natural promoter Waltraud G. Müller, Dawn Walker, Gordon L. Hager, and James G. McNally, Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 Reproduced from The Journal of Cell Biology, 2001, 154(1), 33-48 by copyright permission of The Rockfeller University Press

The amount of transcript produced by the array is correlated with array size. Shown in the top row (a–f) are GFP-GR arrays from different cells fixed at 3 h of 100 nM dexamethasone. The corresponding RNA FISH signals are shown in the middle row and the overlay images in the bottom row. Note that progressive increase in array size (a–f) is accompanied by progressive increase in the RNA FISH signal. This correlation is confirmed by quantitative analysis of 113 cells as shown in the plot at the bottom of the figure. Each point in the plot represents an array, like those in panels a–f, whose total RNA FISH intensity has been measured and plotted as a function of the measured perimeter of the array. Bar, 1 µm.

Request for More information