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Axon™ Digidata® 1550B plus HumSilencer®

The Axon™ Digidata® 1550B plus HumSilencer® is one of the most advanced analog-to-digital signal converters on the market, offering the innovative line synchronous noise-cancelling feature, HumSilencer. This advanced feature learns, adapts, and eliminates local 50/60 Hz line-frequency noise and high-frequency harmonics from incoming signals. The digitizer works with pCLAMPTM 11 Software for data acquisition and analysis. The software supports eight channels of analog acquisition and four acquisition modes.  Intended for precision scientific applications, it is particularly designed for electrophysiology experiments, to send and receive signals from microelectrode amplifiers, and to interact with peripheral instruments such as solution exchangers.

50/60 Hz noise cancellation

HumSilencer is an advanced feature that learns, adapts, and eliminates local 50/60 Hz line-frequency noise patterns and associated high-frequency harmonics from incoming signal up to 20 V peak-to-peak.

Wide Range of Input Signals

Digidata 1550B digitizes a wide range of input signals from -10V to +10V.


Optimized for pCLAMP 11 Software

The pCLAMP Software Suite is the most widely-used patch-clamp electrophysiology data acquisition and analysis program for control and recording of voltage-clamp, current-clamp and patch-clamp experiments.

Noise cancelling

HumSilencer is not a filter and has no effect on acquired signals. It causes no signal distortion such as frequency change, amplitude attenuation, phase shift, or DC voltage change

HumSilencer adaptation

HumSilencer automatically recognizes changes in noise amplitudes and adapts by removing noise in less than one second.

Three configurations

Three configurations are available: Digidata 1550B4 with four HumSilencer channels, Digidata 1550B1 with one HumSilencer channel and Digidata 1550B0 without a HumSilencer channel.

The low digitization noise is maintained in this digitizer. Analog input channel crosstalk is prevented by the use of separate analog-to-digital converters (ADCs) for each of the analog input channels. Additionally, the use of the latest manufacturing processes and precision components contribute to an extremely low-noise 16-bit signal.    The Digidata 1550B comes equipped with up to four analog HumSilencer inputs, thus allowing stimulation and recording of multiple cells at once without line-frequency noise. All of the eight analog input channels can be simultaneously digitized at the highest sampling rate of 500 kHz for maximum throughput. Multiple triggering options are available via hardware and software.






HumSilencer elimination of 60 Hz line frequency noise on four channels. Recordings made from four model cells attached to a MultiClamp 700B amplifier with 60 Hz line-frequency noise introduced by a noise generator placed next to the model cell. Signals were digitized by a Digidata 1550B plus HumSilencer. Black traces: raw data; red traces: same data with HumSilencer enabled.






The HumSilencer quickly learns the noise pattern. Recordings made from a model cell attached to a MultiClamp 700B amplifier with 60 Hz line- frequency noise introduced by a noise generator placed next to the model cell. Signals were digitized by a Digidata 1550B plus HumSilencer. Bottom trace: raw data; top trace: same data with HumSilencer enabled. Vertical green lines indicate the time it takes HumSilencer to learn and eliminate noise (0.78245 s). Even if HumSilencer is only enabled after noise appears, it quickly learns and eliminates the noise once it’s turned on.


Single-channel recordings from a membrane patch excised from an HEK293 cell transfected with the α-subunit of an olfactory cyclic nucleotide-gated channel. Membrane was clamped at +60 mV in the presence of 2 μM cGMP.

An evoked excitatory postsynaptic current recording from a corticostriatal neuron in a brain slice preparation. The stimulation electrode was placed in the layer V/VI region of the cortex. Membrane was clamped at -70 mV..

Action potential recordings from a neuron in an isolated dorsal root ganglia of a rat brain. Action potentials were evoked by injecting a current step of 110 μA. The resting membrane potential was at -50 mV..

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